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26, 27, 28 In these studies, the mean concentration of reduced glutathione (GSH), the primary intracellular antioxidant and redox buffer, was found to be significantly decreased, whereas oxidized glutathione disulfide (GSSG) was significantly increased, resulting in a decrease in the glutathione redox ratio (GSH/GSSG) in both plasma and primary immune cells from children with autism

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The short plasma half-life of GHK-Cu is one reason why twice-daily dosing schedules are sometimes used in injectable protocols, though the peptide's downstream gene expression effects appear to persist well beyond its plasma clearance