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In the double-blind, placebo-controlled, double-crossover trial published in Prostaglandins, Leukotrienes & Essential Fatty Acids, boys diagnosed with ADHD were given acetyl-L-carnitine at 100 mg per kg of body weight per day, divided

Viability assay was performed using CellTiter-Glo luminescent cell viability assay kit (Promega, WI)

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Research suggests GHK-CU has a relatively short half-life, making twice-daily administration theoretically superior for sustained gene expression effects