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HPLC measurement of DA, DOPAC and HVA DA and metabolites were separated by injecting 50 μl of sample into a Microsorb C18 column (100 × 4.6 mm column, Analytical Column, Waters) and a mobile phase containing 130 mM NaH 2 PO 4 , 2 mM NaCl, 0.1 mM EDTA, 2 mM OSA (1-octanesulphonic acid sodium) and 12.5% (v/v) methanol, pH 3.71, at a flow rate of 1 ml min −1

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The Luebering-Rapoport shunt, a side arm of Embden-Meyerhof pathway, produces 2,3-DPG (Figure 1), essential for the regulation of O 2 affinity [1, 2]

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